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Image Search Results
Journal: bioRxiv
Article Title: An open source statistical and data processing toolbox for wide-field optical imaging in mice
doi: 10.1101/2021.04.07.438885
Figure Lengend Snippet: A) User input needed for these steps. Excel sheet updated with mouse information as well as image_system_info.m updated with image system information is read into the pipeline. A single frame is loaded and a user defined binary mask (using the roipoly.m function) and seed locations are created. B) These steps will run without user input. Step-wise illustration of the data pre-processing pipeline color-coded for hemoglobin (red), fluorophore (green), or both (black, grey) specific steps. C) After the pre-processing steps in A,B), the toolbox supports various types of data analysis (e.g., seed-wise FC, bilateral FC, node degree FC, spectral) and all follow the dark blue illustrated analysis pipeline. The initial analysis is performed per run, per mouse, followed by averaging across mice. A pixel-wise statistical comparison is performed (e.g., t-test) and the cluster size-based thresholding is implemented to correct for multiple comparisons. *Only for seed-wise FC are matrices created and fed through the enrichment pipeline (light blue).
Article Snippet: Binary masks are created using the
Techniques: